human egfr gene mutations detection kit  (Beijing ACCB Biotech Ltd)

Journal: Frontiers in Oncology

Article Title: Predicting epidermal growth factor receptor mutation status of lung adenocarcinoma based on PET/CT images using deep learning

doi: 10.3389/fonc.2024.1458374

Figure Lengend Snippet: The relationship between clinical variables of patients and EGFR mutation status (wild-type vs. mutation) in the training set.

Article Snippet: Mutations in EGFR exons 18, 19, 20, and 21 were tested with polymerase chain reaction (PCR)–based amplified refractory mutation system by using the human EGFR gene mutation detection kit of both institutions (LUSH: Beijing SinoMD Gene Detection Technology Co., Ltd., China; Amoy Diagnostics, Beijing, China; SPPH: Beijing SinoMD Gene Detection Technology Co., Ltd., China; Amoy Diagnostics, Xiamen, China).

Techniques: Mutagenesis

Journal: Cancer Imaging

Article Title: Prognostic and predictive value of interstitial lung abnormalities and EGFR mutation status in patients with non-small cell lung cancer

doi: 10.1186/s40644-024-00712-9

Figure Lengend Snippet: Association between Clinical features and EGFR mutation status

Article Snippet: Molecular analysis of the EGFR mutation status was performed using polymerase chain reaction-based amplification-refractory mutation system analysis with the Human EGFR Gene Mutations Detection Kit (Beijing ACCB Biotech, Beijing, China).

Techniques: Mutagenesis, Diagnostic Assay

Journal: Cancer Imaging

Article Title: Prognostic and predictive value of interstitial lung abnormalities and EGFR mutation status in patients with non-small cell lung cancer

doi: 10.1186/s40644-024-00712-9

Figure Lengend Snippet: A 54-year-old male smoker with stage IV adenocarcinoma located in the left lower lobe. Pre-treatment chest CT ( a ) demonstrated ground-glass opacity, reticulation, traction bronchiectasis, and honeycombing predominantly located in bilateral lower lobes, in accordance with subpleural fibrotic ILA. The tumor was located in ILAs and presented the lobulation sign ( a , b ). The patient underwent fine needle aspiration biopsy, with a pathological diagnosis of adenocarcinoma without EGFR mutation. He was died 300 days after the diagnosis of lung cancer. CT, computed tomography

Article Snippet: Molecular analysis of the EGFR mutation status was performed using polymerase chain reaction-based amplification-refractory mutation system analysis with the Human EGFR Gene Mutations Detection Kit (Beijing ACCB Biotech, Beijing, China).

Techniques: Mutagenesis, Computed Tomography

Journal: Cancer Imaging

Article Title: Prognostic and predictive value of interstitial lung abnormalities and EGFR mutation status in patients with non-small cell lung cancer

doi: 10.1186/s40644-024-00712-9

Figure Lengend Snippet: A 69-year-old female nonsmoker with stage I adenocarcinoma located in the right upper lobe. Pre-treatment chest CT demonstrated the tumor with signs of lobulation, spiculation, vacuole and pleural traction ( a ). Ground-glass opacity and reticulation were observed in bilateral lower lobes without subpleural predominance, in accordance with non-fibrotic ILA ( b ). The patient underwent surgery, and a pathological diagnosis of adenocarcinoma with EGFR mutation was made. She was still alive at the end of follow-up. CT, computed tomography

Article Snippet: Molecular analysis of the EGFR mutation status was performed using polymerase chain reaction-based amplification-refractory mutation system analysis with the Human EGFR Gene Mutations Detection Kit (Beijing ACCB Biotech, Beijing, China).

Techniques: Mutagenesis, Computed Tomography

Journal: Cancer Imaging

Article Title: Prognostic and predictive value of interstitial lung abnormalities and EGFR mutation status in patients with non-small cell lung cancer

doi: 10.1186/s40644-024-00712-9

Figure Lengend Snippet: Receiver operating characteristic curves applied to predict EGFR mutation status. The curves are shown for models based on clinical features, tumor CT features, ILA CT features and integrated features. AUC, area under the ROC curve; CT, computed tomography; EGFR, epidermal growth factor receptor; ILA, interstitial lung abnormality; ROC, receiver operating characteristic

Article Snippet: Molecular analysis of the EGFR mutation status was performed using polymerase chain reaction-based amplification-refractory mutation system analysis with the Human EGFR Gene Mutations Detection Kit (Beijing ACCB Biotech, Beijing, China).

Techniques: Mutagenesis, Computed Tomography

Journal: Cancer Imaging

Article Title: Prognostic and predictive value of interstitial lung abnormalities and EGFR mutation status in patients with non-small cell lung cancer

doi: 10.1186/s40644-024-00712-9

Figure Lengend Snippet: Overall survival according to the EGFR mutation status. The log-rank test showed that patients with EGFR mutations had a good overall survival compared to patients without EGFR mutations ( p < 0.001). EGFR, epidermal growth factor receptor

Article Snippet: Molecular analysis of the EGFR mutation status was performed using polymerase chain reaction-based amplification-refractory mutation system analysis with the Human EGFR Gene Mutations Detection Kit (Beijing ACCB Biotech, Beijing, China).

Techniques: Mutagenesis

Journal: International Journal of Nanomedicine

Article Title: Polymer nanofiber-based microchips for EGFR mutation analysis of circulating tumor cells in lung adenocarcinoma

doi: 10.2147/IJN.S157154

Figure Lengend Snippet: Concordance of EGFR mutation sites detected in primary tissues and CTCs from NSCLC patients. Notes: ( A ) Landscape of somatic mutations detected in primary tissues and CTCs from 72 patients with NSCLC. Seven EGFR alterations were detected by targeted sequencing and the results were computed in the mutational landscape. ( B ) The Venn diagrams were used to summarize the similarity of EGFR mutations in primary tissues and CTCs from 72 NSCLC patients. Abbreviations: CTC, circulating tumor cell; EGFR, epidermal growth factor receptor; NSCLC, non-small cell lung cancer; pri., primary tissue; WT, wild type.

Article Snippet: AMPS-PCR was performed according to the protocol of the Human EGFR Gene Mutation Quantitative Detection Kit (fluorescent PCR; Beijing ACCB Biotech Ltd, Beijing, China).

Techniques: Mutagenesis, Sequencing

Journal: International Journal of Nanomedicine

Article Title: Polymer nanofiber-based microchips for EGFR mutation analysis of circulating tumor cells in lung adenocarcinoma

doi: 10.2147/IJN.S157154

Figure Lengend Snippet: EGFR L858R (exon 21) and T790M (exon 20) sequencing of primary tissues and CTCs. Notes: ( A ) PCR of exon 21 was successfully performed and a band of proper size was found with 2% gel electrophoresis. ARM-PCR and Sanger sequencing were used to detect the mutation sites in L858R. ( B ) PCR of exon 20 was successfully performed and a band of proper size was found with 2% gel electrophoresis. ARM-PCR and Sanger sequencing were used to detect the mutation sites in T790M. Abbreviations: ARM-PCR, amplification refractory mutation system PCR; CTCs, circulating tumor cells; EGFR, epidermal growth factor receptor; PCR, polymerase chain reaction.

Article Snippet: AMPS-PCR was performed according to the protocol of the Human EGFR Gene Mutation Quantitative Detection Kit (fluorescent PCR; Beijing ACCB Biotech Ltd, Beijing, China).

Techniques: Sequencing, Nucleic Acid Electrophoresis, Mutagenesis, Amplification, Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: Polymer nanofiber-based microchips for EGFR mutation analysis of circulating tumor cells in lung adenocarcinoma

doi: 10.2147/IJN.S157154

Figure Lengend Snippet: The relationship between acquired resistance induced by continuous gefitinib treatment and EGFR mutation. Notes: ( A ) Course of the disease with CT images and treatment history and driver mutation. ( B ) L858R and T790M mutation detection via ARMS-PCR. Abbreviations: ARMS-PCR, amplification refractory mutation system polymerase chain reaction; CT, computed tomography; CTC, circulating tumor cell; EGFR, epidermal growth factor receptor.

Article Snippet: AMPS-PCR was performed according to the protocol of the Human EGFR Gene Mutation Quantitative Detection Kit (fluorescent PCR; Beijing ACCB Biotech Ltd, Beijing, China).

Techniques: Mutagenesis, Amplification, Polymerase Chain Reaction, Computed Tomography